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Fig. 1 | Respiratory Research

Fig. 1

From: HNRNPC, a predictor of prognosis and immunotherapy response based on bioinformatics analysis, is related to proliferation and invasion of NSCLC cells

Fig. 1

The differentially expressed profiles of 15 m6A-related genes were established in NSCLC, and NSCLC samples were clustered into two subtypes with significantly different immune infiltration and survival time. a The LUAD and LUSC samples were in different clusters before batch effect removal. After SVA-based batch effects elimination, LUAD and LUSC samples were mixed together, indicating the batch effects have been removed. b 15 m6A-related genes were significantly differentially expressed in NSCLC samples compared with that in normal controls. These 15 genes were regarded as DEMGs and the heatmap of the expression levels of DEMGs in the samples was shown. c Compared with normal counterparts, 4 DEMGs (FTO, ZC3H13, METTL14 and RBM15B) were significantly down-regulated in tumor samples, while the remaining 11 genes were upregulated in tumor samples. d Based on the expression level of the DEMGs, NSCLC samples were distinctly classified into two subtypes, defined as subtype 1 and subtype 2 (left). There were 696 samples in subtype 1 and 298 samples in subtype 2. Subtype 1 showed preferentially longer overall survival time than subtype 2 (right). e The infiltration levels of 13 immune cells were significantly different between subtypes. Subtype 1 displayed higher levels of B memory cells, T regulatory cell, gamma delta T cell, resting NK cell, Monocyte, M2 macrophage, activated mast cell, resting mast cell. f Subtype 1 was present with higher stromal score, immune score, and ESTIMATE score. Thus, subtype 1 with higher immune scores was defined as high TIME group, and subtype 2 was defined as the low TIME group. g Total 10 KEGG pathways were predicted to be closely related with high and low TIME. h The expression level of PD-L1 was significantly decreased in NSCLC samples. PD-L1 expression was higher in subtype 1 than that in subtype 2. i PD-L1 consistently exerted negative correlation with YTHDF1, YTHDF2, RBM15B, while showed positively correlation with HNRNPC, IGF2BP3, and METTL15. *P < 0.05, **P < 0.01, ***P < 0.001

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