Fig. 6
From: PKD1 deficiency induces Bronchiectasis in a porcine ADPKD model
PKD1deficiency caused airway smooth muscle cell phenotype switch via ROCK and ERK signaling. (A) Real-time PCR of genes related to smooth muscle phenotypes in 2-year-old pig lungs. n = 3. (B) Representative Western blot and its quantification analyses of α-SMA expression in 2-year-old pig lungs. n = 3. (C) Immunofluorescence staining of α-SMA and Desmin in 2-year-old pig airways. Scale bar: 100 μm. (D) Real-time PCR of ROCK1 and ROCK2 in 2-year-old pig lungs. n = 3. (E) Representative Western blot analyses of RHOA/ROCK and ERK signaling expression in 2-year-old pigs. n = 3. (F) Immunofluorescence staining of p-ERK in 2-year-old pig airways and blood vessels. Scale bar: 100 μm. The bars represent the mean ± SD; *P < 0.05; **P < 0.01; ***P < 0.001.