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Fig. 6 | Respiratory Research

Fig. 6

From: Potential contribution of early endothelial progenitor cell (eEPC)-to-macrophage switching in the development of pulmonary plexogenic lesion

Fig. 6

Intratracheal TNFα installation enhances the formation of plexiform lesions. A Representative photographs showing the morphology of plexiform lesions in the lung of TNFα-treated birds. Plexiform lesion density was expressed as the number of lesions per section/cm2 per section (n = 6). B Western blots and densitometry analyses of Nrf2. Total proteins extracted from lung tissue were analyzed by immunoblotting with anti-Nrf2 antibody. β-actin are shown as loading control. Densitometry data represent the mean ± 95% confidence interval of 4 birds and are representative of 2 separate experiments. C Immunohistochemistry staining of Nrf2 in lung tissue. Expression of endothelial Nrf2 was semi-quantified by measuring the optical density (OD) in 28 pulmonary arterioles randomly selected from 6 birds in each group

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