Fig. 5
From: IL-10 producing regulatory B cells are decreased in blood from smokers and COPD patients
Effect of in vitro CSE-exposure on the capacity of B cells to produce IL-10. The capacity of MACS sorted B cells to produce IL-10 upon CSE-exposure was determined by flow cytometry: A Percentages of IL-10 producing cells within total B lymphocytes in non-stimulated cells and stimulated cells present in medium, 5% cigarette smoke extract (CSE), and 10% CSE, B IL6 + /IL10 + percentages in stimulated cells present in medium, 5% CSE, and 10% CSE. The levels of IL-10 in supernatant obtained after the stimulation period were measured by ELISA: C Levels of IL-10 in cell culture supernatant, and D Correlation between IL10 levels in supernatant determined by ELISA and IL10 + cells determined by flow cytometry