Table 1 Primer sequences used for RT-qPCR
From: MiR-493-5p inhibits Th9 cell differentiation in allergic asthma by targeting FOXO1
Gene | Species | Type | Sequence |
|---|---|---|---|
FOXO1 | Mouse | Forward | GGGTCCCACAGCAACGATG |
| Â | Â | Reverse | CACCAGGGAATGCACGTCC |
IRF4 | Mouse | Forward | CTTTGAGGAATTGGTCGAGAGG |
| Â | Â | Reverse | GAGAGCCATAAGGTGCTGTCA |
IL-9 | Mouse | Forward | ACACCGTGCTACAGGGAGG |
| Â | Â | Reverse | TGGTTGCATGGCTTTTCG |
IRF1 | Mouse | Forward | CTCAGCAGCTCTACCCTACC |
| Â | Â | Reverse | CACTCAGAGAGACTGCTGCT |
Spi1 | Mouse | Forward | TCAGCCAAGCCAGATCAGAA |
| Â | Â | Reverse | AGAGGAGCTGACATTGGCAT |
BATF | Mouse | Forward | GAAGAGCCGACAGAGACAGA |
| Â | Â | Reverse | TCCTCGGTGAGCTGTTTGAT |
miR-493-5p | Mouse | Forward | GCCGAGTTGTACATGGTAGG |
| Â | Â | Reverse | CTCAACTGGTGTCGTGGA |
U6 | Mouse | Forward | CTCGCTTCGGCAGCACA |
| Â | Â | Reverse | AACGCTTCACGAATTTGCGT |
β-actin | Mouse | Forward | GTCCCTCACCCTCCCAAAAG |
| Â | Â | Reverse | GCTGCCTCAACACCTCAACCC |