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Fig. 5 | Respiratory Research

Fig. 5

From: MiR-493-5p inhibits Th9 cell differentiation in allergic asthma by targeting FOXO1

Fig. 5

MiR-493-5p regulates Th9 cells differentiation by targeting FOXO1. To investigate whether miR-493-5p regulates Th9 cell differentiation by targeting FOXO1, the CD4+T cells were co-transfected with miR-493-5p mimic and FOXO1 overexpressing plasmids/interference sequences under the condition of inducing Th9 cell differentiation. A, B We prepared 3 targeted interference sequences for FOXO1, and the knockdown efficiency of FOXO1 interference sequences was detected by RT-qPCR and Western blot. The result showed that the FOXO1-1 siRNA was the best interference sequences, which was used for the following experiments. C The fluorescent tracker show that the FOXO1 overexpression and miR-493-5p overexpression were present in these cells. E The isolated Naïve CD4+T cells were divided into 8 groups, group. D, F–H Western blot: group C–E show that overexpression of FOXO1 can reverse the overexpression of miR-493-5p, and group F–H show that interference of FOXO1 can reverse the knockdown of miR-493-5p. I–K PCR: group C–E show that overexpression of FOXO1 can reverse the overexpression of miR-493-5p, and group F–H shows that interference of FOXO1 can reverse the knockdown of miR-493-5p. **P < 0.01 compared to A group, ##P < 0.01 compared to D group,&&P < 0.01 compared to G group

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