Fig. 7

Co-administration of anti-CD19, anti-CD4 and anti-CD8 yields strong inhibition of inflammation caused by an antigenic rechallenge. C57Bl/6 J mice were administered MSS three times a week for four weeks. Anti-CD19, anti-CD4 and anti-CD8 or their respective control IgGs were given intraperitoneally 2 days after the last MSS exposure. MSS rechallenge was performed 4 days after antibody injection and mice were euthanized 48 h later. A Quantification of neutrophils/ml of BALF. Single cell suspensions from MSS-rechallenged lungs were analyzed using flow cytometry to determine the frequencies of (B) CD19⁺, (C) B220⁺ and (D) CD90⁺CD8⁺ and CD90⁺CD4⁺ cells. Fully-labeled specimens are presented in black and FMOs (from pooled cells from all experimental groups) are in blue or red. The percentages shown in B–D are from fully-labeled specimens. α: anti, MSS: Methanosphaera stadtmanae, BALF: Bronchoalveolar lavage fluid. FMO: Fluorescence Minus One control. Averages ± SEM. n = 4–5 *p < 0.05