Skip to main content
Fig. 5 | Respiratory Research

Fig. 5

From: Co-modulation of T cells and B cells enhances the inhibition of inflammation in experimental hypersensitivity pneumonitis

Fig. 5

Anti-CD4 and anti-CD8 administration reduces experimental HP inflammation caused by an antigenic rechallenge. C57Bl/6 J mice were administered MSS three times a week for three weeks. Anti-CD4 and anti-CD8 or their respective control IgGs were given intraperitoneally 2 days after the MSS exposure. MSS rechallenge was performed 4 days after antibodies injections and mice were euthanized 48 h later. A Quantification of neutrophils/ml of BALF. Single cell suspension from lungs after the MSS rechallenge were analyzed using flow cytometry to determine the frequencies of (B) CD19+ and (C) CD90+CD8+ and CD90+CD4+ cells. Fully-labeled specimens are presented in black and FMOs (from pooled cells from all experimental groups) are in blue or red. The percentages shown in B and C are from fully-labeled specimens. α: anti, MSS: Methanosphaera stadtmanae. BALF: Bronchoalveolar lavage fluid, FMO: Fluorescence Minus One control. Averages ± SEM. n = 4–5 *p < 0.05

Back to article page