Fig. 1
B cells synergize with T cells in the MSS-induced HP model to amplify airway inflammation. A Saline, 5 × 106 B cells, 2.5 × 106 T cells, or the combination of 5 × 106 B cells and 2.5 × 106 T cells were injected intravenously to Rag1-deficient mice. Beginning 48 h after the transfer, mice were exposed i.n. three consecutive days a week for three weeks to 100 µg MSS and euthanized 24 h after the last exposure. Upward arrow indicates time of euthanasia. Quantifications of (B) total leucocytes and (C) differential counts for leucocyte subsets in BALF. I.V.: Intravenous. Mo: Macrophages. Ly: Lymphocytes. Ne: Neutrophils. Eo: Eosinophils. BALF: Bronchoalveolar lavage fluid. MSS: Methanosphaera stadtmanae. Averages ± SEM. n = 3–6. *p < 0.05