Fig. 1

Differential expression pattern of ferret lungs following cigarette smoke exposure. Age and sex-matched ferrets (Mustela putorious furo) were exposed to cigarette smoke or air control for 6 months, after which lung tissues were isolated and RNA was extracted. Total RNA was isolated from lung homogenate and RNA-Seq analysis was performed. A Principal component analysis of the RNA seq data showing the distinct grouping of air control and smoke-exposed ferret lung (experimental) samples. B Hierarchical clustering of differentially expressed genes (DEGs). Studies were performed in two groups of 6 synchronous ferrets, each with 3 males and 3 females per group