Fig. 3
From: Functional changes in long-term incubated rat precision-cut lung slices
Exemplary illustration of hematoxylin–eosin (HE) stained long-term cultivated rat precision-cut lung slices (PCLS) on day 1 and 29. High-power magnification of the respiratory epithelium partly with cilia on day 1 (A) and day 29 (B) without any alterations. (C) shows normal alveolar epithelium on day 1 and (D) normal alveolar epithelium with some rare cell loss within the alveolar septa at day 29. While lung tissue in the cutting area shows no abnormalities on day 1 (E) and on day 29 incubated in standard culture medium (F), marked metaplastic/regenerative changes appear after 29 days of incubation with 10% fetal bovine serum (FBS) (G). The High-power magnification of G indicates metaplastic/regenerative cell groups with prominent nucleoli (H). Scale bars: 50 µm (A, B, C, D, H), 100 µm (E, F, G). Magnifications: 400x (A, B, C, D, H), 200x (E, F, G)