Fig. 1

Metabolic activity of long-term cultivated rat precision-cut lung slices (PCLS). Metabolic activity of tissue PCLS incubated in a standard culture medium (named PCLS in the absence of serum) and others incubated in a culture medium with the addition of 10% fetal bovine serum (FBS) (named PCLS in the presence of serum) was measured using water soluble tetrazolium (WST)-1 assay (optical density (OD) 450 nm, reference wavelength 595 nm). For each timepoint, measurements were performed in duplicates, n = 3. Data is expressed as mean ± standard deviation (SD). Statistics were carried out using the general mixed model analysis (PROC GLIMMIX, SAS 9.4, SAS Institute Inc., Cary, NC, USA), statistical differences to day 1 are indicated by asterisks (*0.01 ≤ p < 0.05; **0.001 ≤ p < 0.01, ***p < 0.001), statistical differences between the two groups (PCLS in the absence vs. PCLS in the presence of serum) are indicated by hashes (^###p < 0.001)