Fig. 4

IL-37 attenuated IL-24-induced bronchial epithelial migration and EMT in vitro. a The effect of IL-24 with or without IL-37 on the migration of BEAS-2B cells was evaluated by Transwell assay. Scale bar = 100 μm (× 100). b Quantification of the Transwell assay showed the number of migrated cells. c Quantification of E-cadherin, vimentin and α-SMA mRNA expression in BEAS-2B cells treated with 100 ng/ml IL-24 for 24 h with or without 100 ng/ml IL-37 pretreatment for 8 h by RT–qPCR. Representative immunoblots (d) and quantification (e) of E-cadherin, vimentin, and α-SMA protein expression in BEAS-2B cells treated with 100 ng/ml IL-24 for 48 h with or without 100 ng/ml IL-37. Bar diagrams and data are presented as the mean ± standard deviation (SD) from three replicate experiments. * vs. control group; ^# vs. IL-24 group. *^,#P < 0.05; **^{, ##}P < 0.01; ***^,###P < 0.001, ****^,####P < 0.0001