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Fig. 4 | Respiratory Research

Fig. 4

From: Cigarette smoke exposed airway epithelial cell-derived EVs promote pro-inflammatory macrophage activation in alpha-1 antitrypsin deficiency

Fig. 4

Characterization of airway epithelial cell-derived EVs. A Nano sight tracking analysis of size and concentration for EVs isolated from conditioned media of control and cigarette smoke-induced EVs. The bold red curves plot the size distribution of EVs. B Morphological characterization of EVs by transmission electron microscopy (black arrows). C Western blotting experiment of the purified EVs showing Calnexin, CD63, TSG101, membrane bound and soluble TNF-\(\alpha\) and pro and active IL-1 \(\beta\). D The levels of cytokines associated with EVs released by small airway epithelial cells

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