Fig. 1

Characterization of normal and AATD macrophages. A The morphology of monocyte-derived macrophages from normal and AATD individuals were visualized under the light microscopy. B The relative expression of AAT mRNA form normal and AATD macrophages. C The intracellular protein levels of AAT in representative macrophages. D Immunofluorescent images of normal and AATD macrophages with the magnification of 20X, demonstrate the intracellular AAT levels using FITC- labeled AAT antibody. E Quantification of the intracellular levels of AAT protein. F Comparison of the cytokine levels in the culture media of normal and AATD macrophages, *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005