Fig. 8

Enhanced fibrotic response in NEU3 treated mice following bleomycin. Sections of lung tissue from mice treated with saline or bleomycin (Bleo) aspiration at day 0, and NEU3 or inactive NEU3 treatment from days 10 to 20, were stained with A–M picrosirius red to show collagen content, N–T collagen VI, or U fibrosis. A–F Male and G–L female mice aspirated with A and G saline (C) at day 0, B and H) saline at day 0 and NEU3 from days 10–20, C and I saline at day 0 and inactive NEU3 from days 10–20, D and J bleomycin (Bleo) at day 0 and saline from days 10 to 20, E and K bleomycin at day 0 and NEU3 (WT) from days 10–20, F and L bleomycin at day 0 and inactive (In) NEU3 from days 10–20. All images are representative of three mice per group. Scale bar is 0.1 mm. Picrosirius red quantification of M male and female mice. N–S Lung sections of male mice stained with anti-collagen VI antibodies. T Collagen VI quantification of male and female mice combined. U Histological quantification of fibrosis. Values are mean ± SEM, n = 6 mice (3 male and 3 female), or n = 3 for male and female mice analyzed separately. *p < 0.05; **p < 0.01 ***p < 0.001 (one-way ANOVA, Bonferroni’s or Sidak’s test)