Fig. 4

NEU3 but not inactive NEU3 induces fibrosis. Sections of lung tissue from naïve mice and mice treated with NEU3 or inactive NEU3 were stained with A–I picrosirius red to show collagen content, and J–O anti-collagen VI antibodies. A Naïve (control), B NEU3 (WT), and C inactive (In) NEU3 treated male mice, and D Naïve (control), E NEU3 (WT), and F inactive (In) NEU3 treated female mice stained with picrosirius red. All images are representative of three mice per group. Bar is 0.1 mm. G–I Picrosirius red quantification of G combined male and female mice, H male and I female mice. J NEU3, and K inactive NEU3 treated male mice, and L NEU3, and M inactive NEU3 treated female mice stained with anti-collagen VI antibodies. N–O Collagen VI quantification of N male and O female mice. Values are mean ± SEM, n = 6 combined mice (3 male and 3 female), or n = 3 for male and female mice analyzed separately. *p < 0.05; **p < 0.01 (one-way ANOVA, Bonferroni’s or Sidak’s test)