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Fig. 2 | Respiratory Research

Fig. 2

From: Propylene glycol, a component of electronic cigarette liquid, damages epithelial cells in human small airways

Fig. 2

PG decreases cell viability in a concentration-dependent manner. Cell viability was evaluated by using CCK-8 and LDH assays. The CCK-8 assay was conducted after 96 h exposure to 0–4% PG or Gly. The LDH assay was conducted after 24 h exposure to 0–4% PG or Gly. A Effect of PG on cell viability by CCK-8. B Effect of Gly on cell viability by CCK-8. C Comparison of cell viability after PG exposure and Gly exposure. D Effect of PG on LDH release. E Effect of Gly on LDH release. Data are expressed as a relative value to control (indicated as a grey bar). Three batches of SAECs were evaluated on two separate occasions. Values are mean ± SEM. Statistical significance was determined using two-way ANOVA. PG propylene glycol, Gly glycerol, CCK-8 cell counting kit-8, LDH lactate dehydrogenase, SAECs small airway epithelial cells, SEM standard error of the mean

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