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Fig. 7 | Respiratory Research

Fig. 7

From: Protective effects of calcyclin-binding protein against pulmonary vascular remodeling in flow-associated pulmonary arterial hypertension

Fig. 7

Decrease in CacyBP/SIP expression in rats aggravated flow-associated PAH and vascular remodeling. a H&E, EVG, and IF showing morphological changes of the pulmonary vascular remodeling in the indicated groups. Scale bars (black: 20 µm; white: 25 µm). b Quantification of hemodynamic and morphological parameters in the indicated groups. The hemodynamic data were recorded and analyzed using the PowerLab data acquisition system. mPAP, PASP, and RVSP of rats were calculated at least five respiratory cycles. Peripheral vessels 50–150 µm in diameter were randomly chosen and examined for morphological analysis. WT% and WA% of 10–15 arterioles were calculated per rat (WT% = [(external diameter–internal diameter)/external diameter × 100]; WA% = [(total area–luminal area)/total area × 100]). RVHI was calculated as the weight ratio of RV/(left ventricle plus atrial ventricular septum). Data are shown as the mean ± SEM (n = 6 for each group). The difference in variables was compared between AAV-GV143 + MCT-AV and AAV-GV143 + sham groups, and between AAV-siCacyBP + MCT-AV and AAV-GV143 + MCT-AV groups. *: AAV-GV143 + MCT-AV vs. AAV-GV143 + sham; #: AAV-siCacyBP + MCT-AV vs. AAV-GV143 + MCT-AV. **P < 0.01, ***P < 0.001; ##P < 0.01, ###P < 0.001. H&E hematoxylin and eosin, EVG Elastic Van-Gieson, IF immunofluorescent, AAVs adeno-associated viruses, CacyBP/SIP calcyclin-binding protein, MCT monocrotaline, AV aortocaval, PAH pulmonary arterial hypertension, mPAP mean pulmonary arterial pressure, PASP pulmonary arterial systolic pressure, RVSP right ventricular systolic pressures, WT% ratio of wall thickness, WA% ratio of wall area, RVHI right ventricular hypertrophy index

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