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Fig. 5 | Respiratory Research

Fig. 5

From: Protective effects of calcyclin-binding protein against pulmonary vascular remodeling in flow-associated pulmonary arterial hypertension

Fig. 5

CacyBP/SIP regulated hPASMC phenotypic switch via the PI3K/AKT signaling pathway. a Phosphorylation level of PI3K/AKT in hPASMCs among groups. Western blots were quantified by calculating the band density normalized to GADPH. The difference in the phosphorylation of each protein was compared between Adv-siCacyBP and Adv-GV119 groups, and between Adv-CacyBP and Adv-GV314 groups. *: Adv-siCacyBP vs. Adv-GV119; #: Adv-CacyBP vs. Adv-GV314. **P < 0.01; ##P < 0.01, ###P < 0.001. b The phosphorylation level of PI3K/AKT and expression of phenotype markers in hPASMCs after using the PI3K/AKT inhibitor LY294002 (30 nM). Western blots were quantified by calculating the band density normalized to GADPH. The difference was compared between Adv-GV119 + LY294002 and Adv-GV119 groups, and between Adv-siCacyBP + LY294002 and Adv-siCacyBP groups. *: Adv-GV119 + LY294002 vs Adv-GV119; #: Adv-siCacyBP + LY294002 vs Adv-siCacyBP. *P < 0.05, **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001. For these analyses, data are shown as mean ± SEM (n = 3 for each group). hPASMC human pulmonary artery smooth muscle cell, CacyBP/SIP calcyclin-binding protein, PI3K phosphoinositide 3-kinase, AKT protein kinase B

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