Fig. 2

CacyBP/SIP expression was significantly decreased in pulmonary arterioles of flow-associated PAH rats. a Representative confocal images of double-label IF staining with CacyBP/SIP and α-SMA (or CD31) in the pulmonary arterioles. Size bars, 25 µm. b Representative IHC images and analysis of CacyBP/SIP expression in the pulmonary arterioles. Scale bars, 20 µm. AOD, calculated as the integrated option density/area, was used to quantify the expression of CacyBP. Data are shown as mean ± SEM (n = 6 for each group). NS = no significance, **P < 0.01, ***P < 0.001. c Expression of CacyBP/SIP in the lung tissues. Western blots were quantified by calculating the band density and then normalized to GADPH. Data are shown as mean ± SEM (n = 6 for each group). **P < 0.01. d Representative IF staining confocal images of α-SMA (or CD31or CacyBP/SIP) in PASMCs and analysis of CacyBP/SIP expression. Size bars, 25 µm. IF immunofluorescent, IHC immunohistochemistry, CacyBP/SIP calcyclin-binding protein, α-SMA alpha smooth muscle actin, CD31 platelet endothelial cell adhesion molecule-1, MCT monocrotaline, AV aortocaval, PAH pulmonary arterial hypertension, AOD average optical density