Fig. 1

CacyBP/SIP expression was significantly decreased in pulmonary arterioles of patients with CHD-PAH. a Representative confocal images of double-label IF staining of CacyBP/SIP and α-SMA (or CD31) in the pulmonary arterioles of CHD-PAH patients. Size bars, 25 µm. b Representative IHC images and analysis of CacyBP/SIP expression in the pulmonary arterioles of patients. Scale bars, 20 µm. AOD, calculated as the integrated option density/area, was used to quantify expression of CacyBP. Data are shown as mean ± SEM (normal control group: n = 12; reversible group: n = 18; irreversible group: n = 4). **P < 0.01, ***P < 0.001. c Correlation between CacyBP/SIP expression and the pathological grade of pulmonary arterioles in patients with CHD-PAH (n = 22). IF immunofluorescent, IHC immunohistochemistry, CacyBP/SIP calcyclin-binding protein, α-SMA alpha smooth muscle actin, CD31 platelet endothelial cell adhesion molecule-1, CHD congenital heart disease, PAH pulmonary arterial hypertension, AOD average optical density