Fig. 9

Endothelial cell-specific Cpt1a knockout mice were susceptible to developing hyperoxia-induced persistent alveolar and vascular simplification. Endothelial cell-specific Cpt1a KO mice and WT littermates (< 12 h old) were exposed to 95% (A–C, H) or 50% (D–G, I) O₂ for 3 days, and allowed to recover in air until pnd14 (F, G) or pnd60 (A–E, H, I). H&E staining was performed to measure Lm and RAC (A–G), while vWF immunofluorescence was carried out to count vessel numbers in the lung (H, I). Scale bar: 100 μm. N = 6. *P < 0.05, **P < 0.01, ***P < 0.001 vs corresponding air group. ^†P < 0.05, ^††P < 0.01, ^†††P < 0.001 vs WT/O₂ using one-way ANOVA followed by Tukey’s multiple comparison test