Fig. 5
From: ZC3H4 regulates infiltrating monocytes, attenuating pulmonary fibrosis through IL-10
Autophagy is involved in the silica-induced reduction in IL-10 release by monocytes. A RT‒qPCR analysis showing that SiO2 stimulation had no effect on cxcl10 expression. B, C As shown in the string map and bubble map, IL-10 plays an important role in autophagy signaling pathways. D Representative Western blot showing the effect of SiO2 on the upregulation of MAP1LC3B, ATG5 and BECN1 in THP-1 cells. E Densitometric analyses of MAP1LC3B levels in five independent experiments; *P < 0.05 vs. the 0 h group. Densitometric analyses of ATG5 levels in five independent experiments; *P < 0.05 vs. the 0 h group. Densitometric analyses of BECN1 levels in five independent experiments; *P < 0.05 vs. the 0 h group. F ELISA analysis showing that the SiO2-induced reduction in IL-10 protein release by THP-1 cells was reversed by the autophagy blocker 3-MA (n = 5), *P < 0.05 vs. the corresponding group in the control group. #P < 0.05 vs. the con group and the SiO2 group. G ELISA analysis showing that the SiO2-induced reduction in IL-10 protein release by THP-1 cells was promoted by the autophagy agonist rapamycin (n = 5), *P < 0.05 vs. the corresponding group in the control group. #P < 0.05 vs. the con group and the SiO2 group