Fig. 4
From: ZC3H4 regulates infiltrating monocytes, attenuating pulmonary fibrosis through IL-10
Decreased IL-10 release is a key factor that affects fibroblast activation, viability and migration. A IL10ra in the NS group and the SiO2 group is shown in the heatmap of the monocyte cluster. B ELISA analysis showing that SiO2 decreased IL-10 protein expression in THP-1 cells (n = 5); *P < 0.05 vs. the 0 h group. C Representative Western blot showing the effect of CM and IL-10 on the specific upregulation of COL1A1 and ACTA2 in fibroblasts. D Densitometric analyses of ACTA2 levels in three independent experiments; *P < 0.05 vs. the control group, *P < 0.05 vs. the CM group. E Densitometric analyses of COL1A1 levels in three independent experiments; *P < 0.05 vs. the control group, *P < 0.05 vs. the CM group. F Representative images from the scratch assay showing that the migration of fibroblasts was increased by CM and IL-10. Scale bar, 20 μm. G Quantification of the scratch gap distance in three independent experiments; *P < 0.05 vs. the corresponding time point in the control group. H CCK-8 assay results showing that fibroblast viability was increased by CM and IL-10; *P < 0.05 vs. the corresponding time point in the control group, #P < 0.05 vs. the corresponding time point in the CM group, n = 3. I Representative images of gel contraction assays showing fibroblasts treated with CM and IL-10. J Gel contraction assay results demonstrating the decrease in fibroblast viability induced by CM and IL-10; *P < 0.05 vs. the control group, #P < 0.05 vs. the CM group, n = 3