Skip to main content
Fig. 3 | Respiratory Research

Fig. 3

From: ZC3H4 regulates infiltrating monocytes, attenuating pulmonary fibrosis through IL-10

Fig. 3

Increased IL-8 expression is not a key factor that affects fibroblast activation, viability and migration. A RT‒qPCR analysis showing that SiO2 stimulation had no effect on TGFB1 expression. B SiO2 induced the expression of 12 inflammatory factors in THP-1 cells. C RT‒qPCR analysis showing that cxcl8 expression was increased in THP-1 cells in response to SiO2 stimulation (n = 5); *P < 0.05 vs. the 0 h group. D ELISA analysis showing that IL-8 protein expression was increased in THP-1 cells in response to SiO2 stimulation (n = 3); *P < 0.05 vs. the 0 h group. E Representative Western blot showing the effect of IL-8 on the upregulation of COL1A1 and ACTA2 in fibroblasts. F Densitometric analyses of COL1A1 protein expression levels in three independent experiments; *P < 0.05 vs. the control group. G Densitometric analyses of ACTA2 protein expression levels in three independent experiments; *P < 0.05 vs. the control group. H Representative images from the scratch assay showing that the migration of fibroblasts was increased by IL-8. Scale bar, 20 μm. I Quantification of the scratch gap distance in three independent experiments; *P < 0.05 vs. the corresponding time point in the control group

Back to article page