Fig. 1

Silica promotes an infiltrating phenotype in monocytes. A Visualization of major classes of cells using t-SNE. Dots, individual cells; color, cell types. One mouse was used for each group. B Cell numbers and relative proportions of monocytes and macrophages are shown as pie charts at 7 d and 56 d. C As shown in the images, the monocyte numbers were decreased or showed no obvious difference in the SiO₂ group compared with the NC group, while macrophage numbers increased in the SiO₂ group. D Cell trajectory analysis of monocytes and macrophages and pseudotime analysis. E Representative Western blot showing that SiO₂ induced CCR2 expression in a time-dependent manner in THP-1 cells. F Densitometric analyses of CCR2 levels from five independent experiments; *P < 0.05 compared with the 0 h group. G Densitometric analyses of ITGAX levels in five independent experiments. H Representative immunocytochemical staining images showing that SiO₂ induced CCR2 expression in THP-1 cells. Scale bar, 100 μm