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Fig. 8 | Respiratory Research

Fig. 8

From: Knockdown of HSP110 attenuates hypoxia-induced pulmonary hypertension in mice through suppression of YAP/TAZ-TEAD4 pathway

Fig. 8

TEAD4 promotes HSP110 transcription by binding to HSP110 promoter. a Schematic representation of the pGL3-basic-HSP110 promoter reporter constructs used in this study. The HSP110 promoter (− 2000/ + 20) and sequential deletion of the HSP110 promoter shorter fragments (− 1000/ + 20, − 500/ + 20) were cloned into the pGL3-basic luciferase vector. b Constructs with the HSP110 promoter fragments were co-transfected with overexpression vector containing TEAD4 or empty vector into HEK293 cells. The luciferase activity was analyzed. Data are means ± SD from 3 biological replicates. *p < 0.05 compared to vector, #p < 0.05 compared to HSP110 promoter fragments − 2000/ + 20 or − 1000/ + 20 under TEAD4 overexpression. c The chromatin immunoprecipitation of the HSP110 promoter with anti-TEAD4 or anti-IgG antibodies was performed using HPASMCs after hypoxic exposure. The sequences containing the TEAD4-binding sites on HSP110 promoter were amplified by PCR and detected by agarose gel. d The nuclear extract was prepared from HPASMCs after hypoxic exposure and biotinylated-HSP110 DNA probe specific to TEAD4-binding site 3 was used for pull-down assay. The protein level of TEAD4 was detected

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