Fig. 7

HSP110 promotes cell proliferation, migration and autophagy through YAP/TAZ-TEAD4 pathway. a HEK293T cells were transfected with the plasmids for overexpressing HSP110-Flag and/or YAP-Myc for 48 h. The cell lysates were immunoprecipitated using anti-Myc antibody. The protein levels of HSP110 and YAP in precipitates and input were detected. b The cell lysates from HPASMCs were immunoprecipitated using anti-YAP antibody. The interaction of YAP and TEAD4 was detected western blot. HPASMCs were infected with adenovirus vector overexpressing YAP (YAP o/e) or empty vector for 48 h, relative mRNA level (c) and protein level (d, e) of YAP in HPASMCs in each group. f–i Protein levels HSP110 and nuclear TEAD4 and quantitative analysis of relative protein level of HSP110 and TEAD4. j Cell proliferation of YAP overexpessing HPASMCs after different time hypoxic exposure. Wound healing assay (k) and quantification of migration ratio (l) of HPASMCs in each group. Scale bars, 200 μm. m Protein levels of LC3I and LC3II and quantitative analysis of relative protein ratio of LC3-II/I in HPASMCs. n HPASMCs were co-infected with YAP o/e and shHSP110-1 for 48 h, the cell proliferation of HPASMCs in each group after hypoxic exposure. Wound healing assay (o) and quantification of migration ratio (p) of HPASMCs in each group. Scale bars, 200 μm. q Protein levels of LC3I and LC3II and quantitative analysis of relative protein ratio of LC3-II/I in HPASMCs. Data are means ± SD from 3 biological replicates. ^#p < 0.05 compared to the vector or hypoxia-shHSP110-1 group