Fig. 1

HSP110 is upregulated in lung tissues of the hypoxia-induced PH in mice. a The RVSP of the normoxia and hypoxia groups in mice (N = 8). b H&E staining of pulmonary arteries in lung tissues in normoxia and hypoxia group (N = 8). Scale bars, 50 μm. c Quantification of ratio of pulmonary arterial medial thickness to total vessel size (media/CSA) (N = 8). Relative mRNA level (d) and protein level (e, f) of HSP110 in pulmonary arteries (N = 8). g Double immunofluorescence staining of YAP (green) and HSP110 (red) in pulmonary arteries (N = 8). Scale bars, 50 μm. White arrows pointed to HSP110 and YAP double-positive cells. h Double immunofluorescence staining of α-SMA (green) and HSP110 (red) in pulmonary arteries (N = 8). Scale bars, 50 μm. White arrows pointed to HSP110 and α-SMA double-positive cells. Data are means ± SD from 8 mice per group. *p < 0.05