Fig. 4

Knockdown of PTPRO contributes to the suppression of the NF-κB pathway activation in HS. a Immunofluorescence staining showed the intracellular localization of NF-κB P65 in the lung tissues. Magnification: 400 × . Scale = 50 μm or 25 μm. White arrow heads show the representative intracellular localization of NF-κB P65. b Expression levels of total IKKα/β and phosphorylated IKKα/β in the rat lung were detected by Western blot and the ratio of p-IKKα/β to total IKKα/β was calculated. c, d Western blot and expression quantification of phosphorylated NF-κB P65. c and nuclear NF-κB P65. d In the lungs were performed. Values are means (n = 6) ± SD. **P < 0.01