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Fig. 1 | Respiratory Research

Fig. 1

From: PTPRO knockdown protects against inflammation in hemorrhage shock-induced lung injury involving the NF-κB signaling pathway

Fig. 1

a Increased expression of PTPRO in the serum of HS patients and lungs of HS rats. RT-qPCR analysis of the expression of PTPRO in the serum of HS patients (n = 7) versus healthy participants (n = 30). b Timeline of the experiments. Rats were subjected to bloodletting by a catheter inserted into the femoral artery. Mean arterial pressure was maintained at 40–50 mmHg for 1 h (shock-period) and then the rats were resuscitated. Three hours after resuscitation, all rats were euthanized for tissue collection. For PTPRO knockdown, the rats were instilled with lentivirus harboring short hairpin RNA (shRNA) targeting PTPRO (LV-shPTPRO) or negative control shRNA (LV-shNC) 48 h before hemorrhage. c, d HS strongly induces the expression of PTPRO in rat lungs. c The relative mRNA expression of PTPRO in the lung of HS rats was detected by RT-qPCR. d Representative blot image and relative PTPRO protein expression in rat lungs were detected by Western blot. e Immunohistochemical staining for PTPRO in rat lungs. Magnification: 200 ×. Scale = 100 μm. f Double immunofluorescence staining of PTPRO and beta IV tubulin or keratin 19. Magnification: 400 × . Scale = 50 μm. White arrows show the representative co-localization. The values represent the mean (n = 6) ± SD. **P < 0.01

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