Fig. 2

Nintedanib reduced C3M and PRO-C3 concentrations in the PCLS supernatant from patients with PF. a Absolute changes in C3M and b PRO-C3 concentrations from PCLS incubated with nintedanib and pirfenidone for 48 h compared with PCLS incubated with medium. Samples were generated by pooling supernatant from two wells, with each well containing two PCLS. Statistical differences between groups were analysed by Kruskal–Wallis test followed by Dunn’s multiple comparison test for nonparametric data. Two data points in part A and three in part B lie outside the plotted axes. C3M neo-epitope of MMP-9 mediated degradation of type III collagen, PCLS precision-cut lung slices, PF pulmonary fibrosis, PRO-C3 ADAMTS-2 mediated release of the N-terminal pro-peptide of type III collagen