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Table 1 Pericyte panel for flow cytometry

From: Chemokine CXCL12 drives pericyte accumulation and airway remodeling in allergic airway disease

Antibody

Target

Clone

Fluorophore

Dilution (μl/100 μl)

Supplier

CD31

Endothelial cells

390

FITC*

2

Biolegend

PerCp-Cy5.5**

1.25

Biolegend

CD45

Pan-leukocytes

30-F11

PerCp-Cy5.5

1.25

Biolegend

CD146

Pericytes

ME-9F1

PE-Cy7

1.25

Biolegend

FITC

1

PDGFRβ

Pericytes

APB5

APC

5

Biolegend

Ter119

Erythrocytes

TER-119

PerCP-Cy5.5

1.25

Biolegend

  1. *Used as part of dump channel with the LEGENDScreenâ„¢
  2. **Used for endothelial cell exclusion in all other experiments