Fig. 3
From: Chemokine CXCL12 drives pericyte accumulation and airway remodeling in allergic airway disease
Pericytes acquire a migratory phenotype following HDM exposure in vivo. Female C57/Bl6 mice (6–8 weeks old) were subjected to intranasal delivery of sterile PBS (10 µl) or house dust mite extract (HDM; 25 µg in 10 µl) 5 days a week for 5 consecutive weeks. A Lungs were processed into a single cell suspension and submitted to flow cytometric analysis. Pericytes were defined as whole, single cells (FSC, SSC), negative for the markers ter119, CD31, and CD45, and positive for the markers CD146 and PDGFRβ. B The proportion of ter119-/CD31-CD45- cells and the median fluorescence intensity of CD146 (C) and PDGFRβ (D) were determined using FlowJo software. n = 14 per group, representative of three independent experiments. E, F Pericytes were also assessed by flow cytometry to determine the median fluorescence intensity of CD13 and podoplanin on pulmonary pericytes using FlowJo software. n = 14 per group, representative of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. FCS: fetal calf serum; FMO: fluorescence minus one control; FSC: forward scatter; HDM: house dust mite; MFI: median fluorescence intensity; PBS: phosphate-buffered saline; SSC: side scatter