Table 1 Relative modification profiling of carbonylation, oxidative and e-cigarettes biohazard chemical modifications among control and treated V79 cell proteomes

I Carbonylation due to direct oxidation of Lys. Thr and Pro amino acid side chains

2-pyrrolidone from Pro

1

0.03

1.8

0.07

1.4

0.07

1

0.04

2-amino-3-oxo-butanoic_acid from Thr

1

0.15

0.04

0.001

0.02

0.004

0.01

0.002

Lysine oxidation to aminoadipic semialdehyde

N/A

0

∞

0.001

N/A

0

N/A

0

II Carbonylation due to Michael addition reaction of α.β-unsaturated aldehydes derived from lipid peroxidation

Levuglandinyl-lysine anhyropyrrole adduct

1

0.01

2.5

0.05

4

0.11

0.7

0.02

4-hydroxynonenal (HNE)

N/A

0

∞

0.001

∞

0.003

N/A

0

III Carbonylation events due to AGEs formation

Carboxymethyl on N term

N/A

0

∞

0.004

∞

0.002

N/A

0

Other direct oxidative modifications

Met single oxidation (sulfoxide)

1

54

0.8

66

0.7

78

0.8

68

Met double oxidation (sulfone)

1

0.02

5.9

0.2

4.6

0.2

1.9

0.06

His and Trp oxidation

1

0.09

5.9

0.77

4.8

0.85

3.7

0.51

Proline oxidation to pyroglutamic acid

N/A

0

N/A

0

∞

0.003

N/A

0

Tryptophan oxidation to hydroxykynurenin

1

0.01

1.2

0.02

0.9

0.02

1.4

0.02

Chemical modification events most likely caused or enhanced by E-cigarette liquid and nicotine

2.3-dihydro-2 2-dimethyl-7-benzofuranol N-methyl carbamate

N/A

0

N/A

0

∞

0.001

N/A

0

5-dimethylaminonaphthalene-1-sulfonyl

N/A

0

∞

0.004

∞

0.001

∞

0.002

Hydroxymethyl

N/A

0

∞

0.0003

N/A

0

N/A

0

O-Isopropylmethylphosphonylation

N/A

0

∞

0.003

N/A

0

N/A

0

Tri nitro benzene

N/A

0

∞

0.1

∞

0.1

N/A

0

Diethylation

N/A

0

∞

0.002

N/A

0

N/A

0

Acetaldehyde + 26

1

0.01

1.8

0.03

1.5

0.04

1.4

0.03

Dichlorination of tyrosine residues

1

0.0004

45

0.03

32

0.03

27

0.02

Ethylation

1

0.01

2.7

0.05

1.4

0.04

3.4

0.07

Replacement of 2 protons by nickel

1

0.08

1.3

0.16

1.1

0.18

1.1

0.14