Association of childhood BMI trajectory with post-adolescent and adult lung function is mediated by pre-adolescent DNA methylation

Rathod, Rutu; Zhang, Hongmei; Karmaus, Wilfried; Ewart, Susan; Mzayek, Fawaz; Arshad, S. Hasan; Holloway, John W.

doi:10.1186/s12931-022-02089-4

Table 2 Indirect and direct effects of childhood BMI trajectories on lung function parameters at post-adolescent and adulthood via pre-adolescent DNAm along with the information on locations of the identified CpG sites

From: Association of childhood BMI trajectory with post-adolescent and adult lung function is mediated by pre-adolescent DNA methylation

Outcome	Sex	CpG sites	Effects at 18 years*				Effects at 26 years**				Genes	Gene Location	Chr. ^#
Outcome	Sex	CpG sites	Indirect eff. ^$	p-value	Direct eff.^¥	p-value	Indirect eff. ^$	p-value^@	Direct eff.^¥	p-value	Genes	Gene Location	Chr. ^#
FEV₁	Males	cg00612625	− 0.16	0.02	− 0.14	0.55	− 0.001	0.99	− 0.57	0.03	HPSE2	Body	10
		cg21883115	− 0.14	0.03	− 0.16	0.50	− 0.04	0.48	− 0.53	0.03	MAGI3	–	1
		cg00509207	− 0.14	0.04	− 0.14	0.52	0.02	0.83	− 0.60	0.01	TSPAN11	TSS1500	12
		cg06710672	− 0.13	0.03	− 0.14	0.54	− 0.15	0.05	− 0.41	0.05	CACNA1H	–	16
	Females	cg09005221	− 0.17	0.03	0.28	0.27	− 0.13	0.16	0.15	0.64	DIO2	Body	14
		cg14654082	0.21	0.04	− 0.09	0.74	0.23	0.07	− 0.16	0.62	CAMTA1	Body	1
		cg13314614	0.28	0.004	− 0.19	0.45	0.15	0.12	− 0.14	0.66	DHRS3	Body	1
		cg19088553	0.16	0.03	− 0.08	0.76	0.17	0.05	− 0.19	0.53	GRIK2	Body	6
		cg19411557	0.25	0.04	− 0.15	0.55	0.16	0.17	− 0.09	0.76	RP11− 285B24.1	–	3
		cg04206296	0.17	0.03	− 0.08	0.76	0.15	0.04	− 0.15	0.63	DEGS1	TSS1500	1
FVC	Males	cg10337540	− 0.12	0.04	− 0.17	0.40	0.01	0.88	− 0.73	0	LHFP	Body	13
		cg00612625	− 0.15	0.02	− 0.14	0.46	− 0.005	0.95	− 0.71	0	HPSE2	Body	10
	Females	cg26606556	− 0.19	0.03	0.40	0.05	0.08	0.4	0.14	0.59	FARSA	TSS200	19
		cg18860868	− 0.14	0.04	0.37	0.07	− 0.12	0.1	0.34	0.19	FBXO21	Body	12
		cg00647820	− 0.16	0.03	0.41	0.04	0.06	0.47	0.16	0.54	DHX58	Body	17
		cg21909286	0.16	0.02	0.07	0.73	0.04	0.68	0.19	0.39	BEGAIN	–	14
FEV₁/FVC	Males	cg15594471	− 0.21	0.02	0.25	0.25	− 0.17	0.14	0.30	0.30	RP11-856F16.2	–	11
		cg15628222	− 0.16	0.03	0.18	0.42	− 0.15	0.12	0.29	0.34	TRAM1	TSS1500	8
		cg18078387	− 0.16	0.03	0.19	0.36	− 0.18	0.14	0.21	0.44	SCIN	TSS200	7
	Females	cg18164362	0.24	0.04	− 0.47	0.14	0.15	0.24	− 0.61	0.13	RP11-163,019.10	–	11
		cg02710571	0.29	0.02	− 0.47	0.09	0.21	0.08	− 0.67	0.04	CCDC140	5’UTR	2
		cg14880800	0.28	0.02	− 0.53	0.05	0.21	0.08	− 0.67	0.02	DNAH5	TSS1500	5
		cg14654082	0.23	0.03	− 0.44	0.12	− 0.08	0.65	− 0.38	0.32	CAMTA1	Body	1
		cg13314614	0.20	0.04	− 0.44	0.11	0.05	0.67	− 0.50	0.17	DHRS3	Body	1
		cg26606556	0.24	0.04	− 0.46	0.10	0.08	0.48	− 0.53	0.18	FARSA	TSS200	19
		cg17023770	0.25	0.02	− 0.49	0.07	− 0.02	0.86	− 0.42	0.26	PCDHB15	TSS1500	5
		cg20015855	0.25	0.04	− 0.46	0.11	0.16	0.09	− 0.61	0.09	CORO2B	Body	15
		cg11763866	0.24	0.04	− 0.46	0.09	0.29	0.04	− 0.78	0.007	PPFIA1	–	11

*For analyses at 18 years, path analyses were adjusted for second-hand smoke exposure before 10 years, socio-economic status (SES), active smoking status at 18 years, height at 18 years, age of pubertal events (age at onset of voice deepening for males and age at onset of menarche for females)
**Analyses at 26 years used similar covariates: second-hand smoke exposure before 10 years, socio-economic status (SES), active smoking status at 26 years, height at 18 years, age of pubertal events (age at onset of voice deepening for males and age at onset of menarche for females)
Underlined CpGs are common CpGs between two lung function parameters in same sex
CpGs in bold font show the same direction of indirect effects at 26 years as those at 18 years
$Indirect eff.: Estimates of indirect effects (coefficients)
¥Direct eff.: Estimates of direct effects (coefficients)
@ In the replication analysis, p-values ≤ 0.05 are in bold font and p-values ≤ 0.1 are underlined
#Chr.: Chromosome

Back to article page

ISSN: 1465-993X

Contact us

General enquiries: journalsubmissions@springernature.com

Respiratory Research

Contact us