Fig. 1

Myeloid Dnmt3b deficiency promotes alternative polarization of bone marrow-derived macrophages (BMDMs). A Relative gene expression of Arg1 (Arginase 1) and Fizz1 (Resistin like alpha) in BMDMs of control (Dnmt3b^fl/fl) and DNMT3B conditional knockout (Dnmt3b^fl/flLysM^cre) mice stimulated with IL4 (20 ng/ml) for 0, 6 and 24 h determined by RT-qPCR. Expression levels are relative to Hprt (hypoxanthine–guanine phosphoribosyltransferase). B Relative gene expression of Pdgfa (platelet derived growth factor subunit A) and Fn1 (Fibronectin) in BMDMs of control (Dnmt3b^fl/fl) and DNMT3B conditional knockout (Dnmt3b^fl/flLysM^cre) mice stimulated with TGFB1 (5 ng/ml) for 0, 6 and 24 h determined by RT-qPCR. Expression levels are relative to Hprt. C Relative gene expression of Il6 (Interleukin 6) and Tnfa (Tumor necrosis factor-α) in BMDMs of control (Dnmt3b^fl/fl) and DNMT3B conditional knockout (Dnmt3b^fl/flLysM^cre) mice stimulated with lipopolysaccharide (LPS, 100 ng/ml) for 0, 2, 6, 12 and 24 h determined by RT-qPCR. Expression levels are relative to Hprt. D IL6 and TNFA protein levels in the supernatant of BMDMs from control (Dnmt3b^fl/fl) and DNMT3B conditional knockout (Dnmt3b^fl/flLysM^cre) mice stimulated with LPS (100 ng/ml) for 0, 2, 6, 12 and 24 h determined by ELISA. Data represent results one of three independent experiments showing similar results. Data are presented as mean ± SEM, n = 6 per group; Black bars: Littermate control mice, open bars: myeloid specific DNMT3B deficient mice. *p < 0.05, **p < 0.01