Fig. 5

ODE-induced lung infiltrates are increased in the setting of CIA in DR4 mice. A Representative H&E-stained lung section images from each treatment group. B–D Lung cell infiltrates were determined by flow cytometry on live CD45⁺ cells after exclusion of debris and doublets. Number of cells was determined by multiplying the percentage of cell subtype by the total number of lung cells enumerated. B Lung lymphocytes defined as CD3⁺CD4⁺ T cells, CD3⁺CD8⁺ T cells, CD19⁺CD11b⁻ B2 B cells, and CD19⁺CD11b⁺ B1 B cell subpopulations. C Lung myeloid cells defined as CD11c⁻Ly6G⁺ neutrophils and D CD11c⁺CD11b^lo alveolar macrophages, CD11c⁺CD11b^hi activated macrophages, and CD11c^intCD11b^hi transitioning monocytes-macrophages. Gating strategies are depicted in Additional file 1: Fig. S1, Additional file 2: Fig. S2. Statistical differences versus saline-treated mice are designated by ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, and ^####p < 0.0001. Lines denote differences between groups (*p < 0.05, **p < 0.01, ****p < 0.0001). N = 5–7 mice/group. Line scale is 100 μm