Fig. 3

A BLOC3-dependent mechanism modulates Myosin IIB level in HPSLF. A and D Western blot analysis of Myosin IIB protein expression in NLF (n = 3 technical replicates) transfected with either control or HPS1 siRNA. β-Actin was used as a loading control (same membranes as in Fig. 1D). Ratio of Myosin IIB to β-Actin density expressed as fold change relative to NLF transfected with control siRNA. B and E Western blot analysis of Myosin IIB protein expression in NLF (n = 3 technical replicates) transfected with either control or HPS4 siRNA. β-Actin was used as a loading control. Ratio of Myosin IIB to β-Actin density expressed as fold change relative to NLF transfected with control siRNA. C and F Western blot analysis of Myosin IIB in NLF (n = 3 technical replicates) transfected with either control or Rab32 siRNA. β-Actin was used as a loading control. The ratio of Myosin IIB to β-Actin density expressed as fold change relative to NLF transfected with control siRNA. Data are expressed as mean ± SEM of three independent experiments. Data were analyzed using a student’s t-test *P < 0.05, **P < 0.01, ****P < 0.0001