Fig. 3
From: Autophagy regulates the effects of ADSC-derived small extracellular vesicles on acute lung injury
Autophagy mediated the effects of ADSC-sEVs on tight junction-associated protein expression in LPS-treated PMVECs. A Representative western blots showing ZO-1 and claudin-5 expression in PMVECs. B Statistical analysis of ZO-1 and claudin-5 expression in PMVECs. LPS decreased the expression of ZO-1 and claudin-5 in PMVECs, which was significantly inhibited by ADSC-sEVs treatment. Autophagy inhibition weakened the effect of ADSC-sEVs on the expression of ZO-1 and claudin-5 (the relative expression of claudin-5, control, 0.95 ± 0.02 vs. LPS, 0.47 ± 0.01 vs. LPS + ADSC-sEV, 0.73 ± 0.03, LPS + ADSCsiATG5-sEV, 0.51 ± 0.01. the relative expression of ZO-1, control, 1.29 ± 0.05 vs. LPS, 0.75 ± 0.02 LPS + ADSC-sEV, 1.05 ± 0.03 vs. LPS + ADSCsiATG5-sEV 0.84 ± 0.04). The results are expressed as the mean ± SD of three independent experiments