Fig. 5

AT7519, a CDK inhibitor, reduces NETs in LPS-induced acute lung injury (ALI). A, B Western blotting was used to determine the Cit-H3 level, n = 3; The immunoblot data currently presented as separate groups were run on the same gel (with non-essential lanes removed). C PicoGreen assay was used to determine the cf-DNA/MPO level in the BALF, n = 5. D Confocal microscopy is used to detect MPO/Cit-H3 levels in lung tissue. p values < 0.05 indicated statistical significance