Fig. 3
CSE treatment induces 6-keto PGF1α/TXB2 imbalance and elicits opposing effects on PGE2 production in human PASMCs and PAECs. Confluent human PASMCs (A, C, E, and G) and PAECs (B, D, F, and H) were treated with different concentrations of CSE for 72 h and 24 h, respectively. Medium was collected, and levels of PGE2 (A, B), 6-keto PGF1α (C, D) and TXB2 (E, F) were determined by ELISA and 6-keto PGF1α/TXB2 ratio (G and H) was calculated. Results were normalised with total cell protein and are expressed as pg/mg protein (A–F) or 6-keto PGF1α/TXB2 ratio (G, H). Each data point represents mean ± SEM from three independent experiments using PASMCs (A, C, E and G) from three different donors and PAECs (B, D, F, and H) from one donor. *P < 0.05, **P < 0.01, ***P < 0.001 compared with corresponding untreated cells