Fig. 2

Changes in profibrotic endpoints. A Representative images of Masson’s trichrome-stained mouse lung sections taken at ×100 magnification, scale bar = 250 μm. Blue staining around the airways indicates positive trichrome staining. B Quantification of mean Masson’s trichrome staining, n = 12–18 mice per group, 10 airways per mouse. C Mean percent invasion in control treated fibroblasts (number of invading cells divided by number of migrating cells × 100) lung fibroblasts determined by Matrigel invasion assay, n = 10–12 mice per group, cultures performed in duplicate. D Col1a1 mRNA expression in whole lung, n = 5–8 mice per group. E Total TGF-β1 protein levels in BAL n = 12–18 mice per group; *p < 0.05, **p < 0.01, ****p < 0.0001 vs. corresponding PBS treatment, ^##p < 0.01 vs. saline by two-way ANOVA with Šidák correction