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Fig. 3 | Respiratory Research

Fig. 3

From: Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease

Fig. 3

CSE enhanced HE4 expression in HBE cells but not in HPF cells. A Different concentrations of CSE increased HE4 mRNA expression in HBE cells (n = 3). B, C The image of western blot was shown and the band intensity was quantified by ImageJ (n = 3). Compared with controls, 5% CSE and 10% CSE markedly enhanced HE4 protein expression in HBE cells. D The secretory HE4 levels were detected in collected CSE-exposed HBE supernatants using ELISA (n = 4). E, F CSE had no obvious impact on HE4 expression in HPF cells (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences relative to controls. CSE, cigarette smoke extract; HE4, human epididymis protein 4; HBE, human bronchial epithelial; HPF, human pulmonary fibroblast

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