Fig. 5
From: The role of retinoid-related orphan receptor-α in cigarette smoke-induced autophagic response
RORα directly interacts with p53 and increases p53 transcriptional activity. A Beas-2B cells were exposed to the indicated concentration of CSE for 16 h. B Co-immunoprecipitation of RORα and p53 at the endogenous level in Beas-2B cells treated with CSE for 4 h. C A p53 reporter assay was performed on the lung fibroblasts from RORαWT and RORαsg/sg mice treated with the indicated concentration of CSE. *p < 0.05 compared with 0% CSE treatment in WT fibroblasts, and #p < 0.05 compared with WT fibroblasts. D H1299 cells were transfected with a mock or p53 WT plasmid for 24 h and exposed to 20% CSE for 4 h. Total cell extracts were subjected to western blot using anti-RORα, anti-DRAM, anti-p62, anti-LC3, and anti-α-actinin antibodies