Fig. 2
From: The role of retinoid-related orphan receptor-α in cigarette smoke-induced autophagic response
Effect of RORα on CSE-induced autophagy in Beas-2B cells. A Beas-2B cells were exposed to 20% CSE for the indicated time. B Cells were exposed to the indicated concentration of CSE for 4 h. Under these conditions, the total cell extracts were subjected to western blot assays using anti-RORα, anti-p62, anti-LC3, and anti-α-actinin antibodies. C Beas-2B cells were transfected with the control or RORα siRNAs for 48 h. The cell lysates were prepared after treatment with 20% CSE for 24 h and subjected to western blot. D Under the same conditions as in C, the cells were analyzed for their viability using an MTT assay. The data are expressed as the mean ± S.E. of 2 independent experiments with quadruplicate determinations. *p < 0.01