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Fig. 1 | Respiratory Research

Fig. 1

From: The role of retinoid-related orphan receptor-α in cigarette smoke-induced autophagic response

Fig. 1

RORα regulates CSE-induced autophagy in a mouse model. A Mice were exposed to smoke for 5 days/week for 24 weeks. Three mice were assigned to each group. The histopathologic features of lungs are shown by H&E stain. B Homogenates of mouse lung tissues were subjected to western blot assays using anti-RORα, anti-DRAM, anti-p62, anti-LC3, anti-p53, and anti-β-actin antibodies. Densitometry was used to analyze the fold changes of the levels of C RORα, D DRAM, E LC3B, and F p62. * and ** indicate p < 0.05 and p < 0.01, respectively. G RORαWT and RORαsg/sg mice were exposed to smoke for 5 days/week for 24 weeks. Homogenates of mouse lung tissues were subjected to western blot assays using anti-p62 and anti-β-actin antibodies. Representative blots of proteins in the lung tissues are shown. H Densitometry was used to analyze the fold changes of the levels of p62 for four groups of mice: RORαWT + Con group (n = 4), RORαWT + Smoke group (n = 10), RORαsg/sg + Con group (n = 4) and RORαsg/sg + Smoke group (n = 7). *p < 0.05 compared with the RORαWT mice of the nonsmoking (RORαWT + Con) group, and #p < 0.01 compared with the RORαWT mice of the smoking (RORαWT + Smoke) group

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