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Fig. 2 | Respiratory Research

Fig. 2

From: Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial–mesenchymal transition during radiation-induced pulmonary fibrosis

Fig. 2

IR downregulates miR-486-3p to promote EMT. A qRT-PCR was performed for verifying the transfection efficiency of miR-486-3p inhibitor in A549 cells. B EMT-related protein was checked by Western Blot. On the right, bar graph shows the quantitative analysis of the protein using ImageJ. C qRT-PCR was performed for verifying the transfection efficiency of miR-486-3p inhibitor in BEAS-2B cells. D EMT-related protein was checked by Western Blot in BEAS-2B cells. On the right is the protein quantitative protein mapping. E Transfection efficiency of miR-486-3p mimic in A549 cells and BEAS-2B cells (G). Detection of EMT-related proteins after transfected miR-486-3p mimic in A549 (F) and BEAS-2B cells (H), and the bar graph showed gray value analysis. *p < 0.05 compared with NC, #p < 0.05 compared with IR group.

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