Fig. 7

LV-Chop-shRNA reduced pulmonary fibrosis and fibroblast activation. A Pulmonary fibrosis was determined by hematoxylin–eosin (H&E) staining and Masson’s trichrome staining. Representative images of three independent experiments are shown. Bar = 100 μm. B Quantification of pulmonary fibrosis by Ashcroft score. C Quantification of pulmonary fibrosis by measuring fibrotic area in lung sections with Masson’s trichrome staining. D Quantification of pulmonary fibrosis by measuring hydroxyproline of mouse lung. E Expression of proteins involved in ER stress, HH signaling pathway, and fibroblast activation was examined with Western blotting. β-actin was used as the loading control. F Quantification of protein levels relative to control. # indicates significance in the comparison between bleo(TM + shChop) and bleo(TM) group. ns, non-significant. G Expression of Fibronectin after adding GANT61 in mouse lung. (H) Quantification of protein levels relative to control